Complete 1H,13C,15N assignments of zinc Clostridium pasteurianum rubredoxin

Two forms of the protein, one is identical to the crystal structure The second form contains a formyl methionine instead of a methionine in position one

Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Mon Jul 25 14:49:33 2011

Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Mon Jul 25 12:24:02 2011

(2S)-2-formamido-4-methylsulfanyl-butanoic acid

Hydrogen bonds in rubredoxins from mesophilic and hyperthermophilic organisms.

The extent and strength of the hydrogen bond networks in rubredoxins from the hyperthermophile Pyrococcus furiosus (PfRd), and its mesophilic analogue Clostridium pasteurianum (CpRd), are examined and compared using NMR spectroscopy. NMR parameters examined in this study include through-hydrogen bond (h3)J(NC)(') scalar couplings and (1)H, (13)C, and (15)N chemical shifts, as well as covalent (1)J(NH) and (1)J(NC)(') scalar couplings. These parameters have allowed the characterization in solution of 12 hydrogen bonds in each protein. Despite a 83% sequence homology and a low RMSD for the backbone heavy atoms (0.648 A) in the crystalline state, subtle, but definite, changes have been identified in the detailed hydrogen-bonding patterns. CpRd shows an increased number of hydrogen bonds in the triple-stranded beta-sheet and an additional hydrogen bond in the multiple-turn segment including residues 14-32. On the other hand, PfRd exhibits an overall strengthening of N-H...O=C hydrogen bonds in the loops involved at the metal binding site as well as evidence for an additional NH...S(Cys) hydrogen bond involving the alanine residue 44. These data, as well as temperature dependence of the NMR parameters, suggest that the particular NMR hydrogen bond pattern found in the hyperthermophile rubredoxin leads to an increased stabilization at the metal binding pocket. It seems to result from a subtle redistribution of hydrogen-bonding interactions between the triple-stranded beta-sheet and the actual metal binding site.
Hydrogen bonds in Rubredoxins from mesophilic and hyperthermophilic organisms, Biochemistry, in preparation

Clostridium Pasteurianum Rubredoxin G10a Mutant

Clostridium Pasteurianum Rubredoxin G43a Mutant

Clostridium Pasteurianum Rubredoxin C42s Mutant

Solution Structure Of Reduced Clostridium Pasteurianum Rubredoxin, Nmr, 20 Structures

X-Ray Crystal Structure Of Oxidized Rubredoxin

X-Ray Crystal Structure Of Reduced Rubredoxin

Rubredoxin (zn-substituted) At 1.2 Angstroms Resolution

Rubredoxin (Oxidized, Fe(Iii)) At 1.1 Angstroms Resolution

Crystal Structure Of Cp Rd L41a Mutant In Oxidized State

Crystal Structure Of Cp Rd L41a Mutant In Reduced State 1 (Drop-Reduced)

Crystal Structure Of Cp Rd L41a Mutant In Reduced State 2 (Soaked)

Crystal Structure Of The Ccr5 Chemokine Receptor

rubredoxin [Clostridium pasteurianum DSM 525 = ATCC 6013]

Rubredoxin domain containing protein [Clostridium pasteurianum DSM 525 = ATCC 6013]

RecName: Full=Rubredoxin; Short=Rd [Clostridium pasteurianum]

A Chain A, Crystal Structure Of V44g CpRubredoxin

A Chain A, Crystal Structure Of V44l CpRubredoxin

A Chain A, Crystal Structure Of Cp Rd L41aMutant In Reduced State 1 (Drop-Reduced)

A Chain A, Crystal Structure Of Cp Rd L41aMutant In Reduced State 2 (Soaked)

A Chain A, Crystal Structure Of Cp Rd L41aMutant In Oxidized State

A Chain A, Clostridium PasteurianumRubredoxin G43a Mutant

A Chain A, Clostridium PasteurianumRubredoxin C42s Mutant

A Chain A, Nickel-Substituted Rubredoxin

A Chain A, Clostridium PasteurianumRubredoxin G10a Mutant

A Chain A, Cobalt-Substituted Rubredoxin

A Chain A, Cadmium-Substituted Rubredoxin

A Chain A, Gallium-Substituted Rubredoxin

A Chain A, Mercury-Substituted Rubredoxin

A Chain A, Rubredoxin (Zn-Substituted) At1.2 Angstroms Resolution

A Chain A, Rubredoxin (Oxidized, Fe(Iii))At 1.1 Angstroms Resolution

A Chain A, X-Ray Crystal Structure OfOxidized Rubredoxin

A Chain A, X-Ray Crystal Structure OfReduced Rubredoxin

A Chain A, Solution Structure Of ReducedClostridium Pasteurianum Rubredoxin, Nmr, 20 Structures

CpRdFe(II) and CpRdFe(III) 1H/2H isotopic shifts

CpRdFe(II) and CpRdFe(III) 15N hyperfine shifts

CpRdFe(III) 1H-15N and 1H-13C dipolar shifts

CpRdFe(II) 1H-15N and 1H-13C dipolar shifts