1H Chemical Shift Assignments and Interproton 3JNHA Coupling Constants of Raphanus sativus Antifungal Protein 1 (Rs-AFP1), a Plant Defensin Isolated from Seeds of Radish

The first residue is named here PCA according to the PDB accession code for pyroglutamic acid and in the PDB coordinates file PYR.

Determination Of The Three-Dimensional Solution Structure Of Raphanus Sativus Antifungal Protein 1 (Rs-Afp1) By 1h Nmr, 20 Structures

Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Mon Jun 20 12:29:48 2011

Fant, F., Vranken, W. F., Martins, J. C., and Borremans F. A.M., "1H Chemical Shift Assignments and Interproton 3JNHA Coupling Constants of Raphanus sativus Antifungal Protein 1 (Rs-AFP1), a Plant Defensin Isolated from Seeds of Radish," Bull. Soc. Chim. Belg. 106, 51-57 (1997).
Determination of the three-dimensional solution structure of Raphanus sativus antifungal protein 1 by 1H NMR.

3JHNHA coupling constants were measured at two transforms, one phased in pure absorption
and the other in pure dispersion, of the DQF-COSY spectrum. From these values the real
coupling constants were calculated according to Kim, Y. and Prestegard, J.H. (1989)
J. Magn. Reson. 84, 9-13

The peptide bond between residues 40 and 41 is in the cis configuration

Determination Of The Three-Dimensional Solution Structure Of Raphanus Sativus Antifungal Protein 1 (Rs-Afp1) By 1h Nmr, 20 Stru

thionin, partial [Brassica oleracea var. viridis]

anther-specific protein homologue [Arabidopsis thaliana]

antifungal protein 1 preprotein [Raphanus sativus]

antifungal protein 1, Rs-AFP1 {N-terminal} [Raphanus sativus=radishes, seeds, Peptide Partial, 44 aa]

antifungal protein 1, AFP1 {N-terminal} [Brassica rapa, Peptide Partial, 27 aa]

antifungal protein 1, AFP1 {N-terminal} [Brassica napus, Peptide Partial, 30 aa]

antifungal protein [Arabidopsis thaliana]

PLANT DEFENSIN 1.1 [Arabidopsis thaliana]

hypothetical protein ARALYDRAFT_476731 [Arabidopsis lyrata subsp. lyrata]

hypothetical protein EUTSA_v10019396mg [Eutrema salsugineum]

PREDICTED: defensin-like protein 1, partial [Brassica rapa]

PREDICTED: defensin-like protein 1 [Brassica rapa]

RecName: Full=Defensin-like protein 13; AltName: Full=Anther-specific protein S18 homolog; AltName: Full=Cysteine-rich antifung

RecName: Full=Defensin-like protein 1; AltName: Full=Cysteine-rich antifungal protein 1; Short=AFP1

RecName: Full=Defensin-like protein 1; AltName: Full=Cysteine-rich antifungal protein 1; Short=AFP1; AltName: Full=M1

RecName: Full=Defensin-like protein 1; AltName: Full=Cysteine-rich antifungal protein 1; Short=AFP1; Flags: Precursor

Natural, i.e. Isolation from the seeds of radish and purification by S-Sepharose cation exchange and RP-HPLC.