Backbone 1H, 13C and 15N chemical shift assignments for the binary L28F ecDHFR:NADPH complex

NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

[[(2R,3S,4R,5R)-5-(3-aminocarbonyl-4H-pyridin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl] [(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate

separate tube (no insert) similar to the experimental sample tube

Journal of the American Chemical Society
Defining the Structural Basis for Allosteric Product Release from E. coli Dihydrofolate Reductase Using NMR Relaxation Dispersion.

DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Backbone 1H, 13C and 15N chemical shift assignments for the ternary L28F ecDHFR:TETRAHYDROFOLATE:NADP+ complex

Backbone 1H, 13C and 15N chemical shift assignments for the ternary L28F ecDHFR:FOLATE:NADP+ complex

Samples contain 1mM 15N,13C-labeled L28F ecDHFR, 10mM NADPH, 5mM ascorbic acid, 10mM glucose-6-phosphate, 20 units/ml Leuconostoc mesenteroides glucose-6-phosphate dehydrogenase, 1mM dithiothreitol, 25mM KCl, 70mM KPi at pH 7.6.

Leuconostoc mesenteroides glucose-6-phosphate dehydrogenase

The CCPN NMR assignment and data analysis application

Department of Biochemistry, Cambridge CB2 1GA, UK