wwPDB validation report of PDB entry 7Z9Q

Complex between Spike and nanobody H11-A10

Severe acute respiratory syndrome coronavirus 2

The images were collected as 50 frame movies

SPT Labtech prototype 300 mesh 1.2/2.0 nanowire grids with a highly reproduceable rectangular bar cross-section were used. The grids were glow-discharged on low for 260 s (Plasma Cleaner PDC-002-CE, Harrick Plasma) to activate the nanowires. Approximately 6 nL of the complex were applied to the grids using a Chameleon EP system (SPT Labtech) at 80% relative humidity and ambient temperature.

S glycoprotein,E2,Peplomer protein,Collar protein,Whisker antigen control protein

7Z6V contains the same Spike protein but complexed to a different nanobody, H11.

CRYO-EM STRUCTURE OF SARS-COV-2 SPIKE : H11-A10 nanobody complex

Hydrogens have been added in their riding positions
Local NCS retraints between domains: 1 2
Local NCS retraints between domains: 3 4
Local NCS retraints between domains: 5 6
Local NCS retraints between domains: 7 8
Local NCS retraints between domains: 9 10
Local NCS retraints between domains: 11 12
Calculated fsc threesigma indicator curve
Author provided fsc threesigma indicator curve
Author provided fsc halfbit indicator curve
Author provided fsc onebit indicator curve
Author provided fsc 0.333 indicator curve
Author provided fsc 0.143 indicator curve
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)