wwPDB validation report of PDB entry 2Y4L

C-TERMINAL TRUNCATION OF LAST 15 RESIDUES

MANNOSYLGLYCERATE SYNTHASE IN COMPLEX WITH LACTATE

DISSECTION OF MANNOSYLGLYCERATE SYNTHASE: AN ARCHETYPAL MANNOSYLTRANSFERASE

MANNOSYLGLYCERATE SYNTHASE IN COMPLEX WITH GDP -MANNOSE

DISSECTION OF MANNOSYLGLYCERATE SYNTHASE: AN ARCHETYPAL MANNOSYLTRANSFERASE RELATED ENTRIES

MANNOSYLGLYCERATE SYNTHASE IN COMPLEX WITH MG-GDP

ENGINEERED RESIDUE IN CHAIN A, GLN 201 TO ALA ENGINEERED RESIDUE IN CHAIN A, GLN 202 TO ALA ENGINEERED RESIDUE IN CHAIN B, GLN 201 TO ALA ENGINEERED RESIDUE IN CHAIN B, GLN 202 TO ALA

ETHYLENE GLYCOL (EDO): ETHYLENE GLYCOL WAS THE CRYSTAL
CRYOPROTECTANT.
MALONATE (MLI): 0.4 M NA MALONATE WAS THE PRECIPITATING
AGENT USED DURING CRYSTALLIZATION.
GUANOSINE-5'-DIPHOSPHATE (GDP): GDP IS THE NUCLEOTIDE
PRODUCT FOLLOWING MGS CATALYZED MANNOSYL TRANSFER.
MANGANESE (MN): 0.2 M MNCL2 WAS PRESENT IN THE MOTHER
LIQUOR DURING CRYSTALLIZATION.

C-TERMINAL TRUNCATION OF LAST 15 RESIDUES. Q201 AND Q202 WERE BOTH MUTATED TO ALANINES FOR SURFACE ENTROPY MINIMIZATION.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY.

MANNOSYLGLYCERATE SYNTHASE (E.C.2.4.1.217)
MANNOSYLGLYCERATE SYNTHASE IN COMPLEX WITH Manganese and GDP

The largest off-origin peak in the Patterson function is 2.76% of the height of the origin peak. No significant pseudotranslation is detected.

20191225.v01 (using entries in the PDB archive December 25th 2019)

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures