wwPDB validation report of PDB entry 2XTW

TOPISOMERASE POISON RESISTANCE FACTOR, RESIDUES 13-226

STRUCTURE OF QNRB1 (R167E-TRYPSIN TREATED), A PLASMID- MEDIATED FLUOROQUINOLONE RESISTANCE PROTEIN

STRUCTURE OF QNRB1 (M102R-TRYPSIN TREATED), A PLASMID- MEDIATED FLUOROQUINOLONE RESISTANCE PROTEIN

CLONED INTO A CLEAVABLE PET28 VECTOR WITH CLEAVABLE HIS6 TAG. PROTEIN IS CLEAVED LEAVING GSH ON N TERMINUS. PROTEIN WAS CLONED STARTING FROM 2ND MET OF THE GENBANK SEQUENCE.

Structure of QnrB1 (Full length), a plasmid-mediated fluoroquinolone resistance protein

The analyses of the Patterson function reveals a significant off-origin peak that is 43.96 % of the origin peak, indicating pseudo-translational symmetry. The chance of finding a peak of this or larger height by random in a structure without pseudo-translational symmetry is equal to 1.6341e-04. The detected translational NCS is most likely also responsible for the elevated intensity ratio. See the relevant section of the logfile for more details.

20191225.v01 (using entries in the PDB archive December 25th 2019)

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures