wwPDB validation report of PDB entry 4X1H

C-terminal derived peptide of guanine nucleotide-binding protein G(t) subunit alpha-1

The C-terminal derived peptide of guanine nucleotide-binding protein G(t) is derived from 340-350 positions in the transducin alpha subunit utilizing a phage display library derived from the C terminal region of transducin (Aris, L, et al 2001) with sequence "IKENLKDCGLF". The construct in this structure has the following sequence: "VLEDLKSCGLF." Hence there are the following mutations I340V, K341L, N342D, and D346S.

Derived from 3CAP, extended to higher resolution

bovine opsin , C-terminal derived peptide of guanine nucleotide-binding protein G(t) subunit alpha-1
Opsin/G(alpha) peptide complex stabilized by nonyl-glucoside

The largest off-origin peak in the Patterson function is 4.85% of the height of the origin peak. No significant pseudotranslation is detected.

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)