wwPDB validation report of PDB entry 5W8S

Lipid-A-disaccharide synthase (E.C.2.4.1.182)
Lipid A Disaccharide Synthase (LpxB)-7 solubilizing mutations

Enzymatic activity assay showed that LpxB with mutations in the swapped portion of the dimer can be complemented by LpxB with mutation in the unswapped portion by forming an LpxB mutant heterodimer with more activity than individual LpxB mutants. Thus, C-terminal swapped dimerization occurs and creates one intact active site in each LpxB mutant heterodimer.

The largest off-origin peak in the Patterson function is 4.29% of the height of the origin peak. No significant pseudotranslation is detected.

20191225.v01 (using entries in the PDB archive December 25th 2019)

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures