wwPDB validation report of PDB entry 3VAF

DNA 5'-D(*UP*UP*(BRU)P*(BRU)P*UP*UP*U)-3'

U2 auxiliary factor 65 kDa subunit, hU2AF(65), hU2AF65, U2 snRNP auxiliary factor large subunit

PROTEIN WAS COCRYSTALIZED WITH A DNA 7MER (5'-D(*UP*UP*(BRU)P*UP*UP*UP*U)-3') BUT THE PROTEIN BOUND THE DNA IN DIFFERENT REGISTERS RESULTING IN THE BRU APPEARING IN MORE THAN ONE POSITION IN THE STRUCTURE

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Splicing factor U2AF 65 kDa subunit/DNA complex
Structure of U2AF65 variant with BrU3 DNA

The largest off-origin peak in the Patterson function is 5.52% of the height of the origin peak. No significant pseudotranslation is detected.

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)