wwPDB validation report of PDB entry 3THT

Alkylated DNA repair protein alkB homolog 8

Probable alpha-ketoglutarate-dependent dioxygenase ABH8

Alkylated DNA repair protein alkB homolog 8 (E.C.1.14.11.-)
Crystal structure and RNA binding properties of the RRM/AlkB domains in human ABH8, an enzyme catalyzing tRNA hypermodification, Northeast Structural Genomics Consortium Target HR5601B

The light scattering data of this construct without C-tag shows mainly monomeric protein in the solution, while the same construct with C-tag appears to have population of monomer and tetrameric species. Consistent with the latter observation, the crystal structure reveals that a few residues of the C-tag play an important role in protein tetramerization, which is possibly not a physiological phenomenon

The largest off-origin peak in the Patterson function is 5.08% of the height of the origin peak. No significant pseudotranslation is detected.

structure factor analysis and validation
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)