wwPDB validation report of PDB entry 3SPE

Protease Resistant Fragment of Gene Product 29 (GP29PR) (UNP residues 96-390)

Crystal structure of the tail sheath protein protease resistant fragment from bacteriophage phiKZ

This structure is a protease resistant fragment, gp29PR, that is a part of a larger full length protein gp29 (this structure is 96-390 out of 1-695). The dimer surface between monomers forming the crystal dimer (identified by PISA) would be not available for interaction in the full length gp29 protein. Therefore, the full length protein cannot form a similar dimer due to steric clashes. So this dimer seems to be an artifact present only for the smaller fragment, gp29PR, and is not biologically relevant.

chain B and (resid 147:177 or resid 184:214 or resid 219:238 or resid 247:287 or resid 300:350)

chain A and (resid 147:177 or resid 184:214 or resid 219:238 or resid 247:287 or resid 300:350)

The largest off-origin peak in the Patterson function is 14.20% of the height of the origin peak. No significant pseudotranslation is detected.

20191225.v01 (using entries in the PDB archive December 25th 2019)

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures