wwPDB validation report of PDB entry 1SP4

LEU-GLY-MEU. EPO is linked to the LEU-GLY-MEU as well as LEU-PRO.
methyl N-[(2S)-4-{[(1S)-1-{[(2S)-2-carboxypyrrolidin-1-yl]carbonyl}-3-methylbutyl]amino}-2-hydroxy-4-oxobutanoyl]-L-leucylglycylglycinate

THE INHIBITOR IS A TWO HEADED EPOXYSUCCINYL INHIBITOR. THE EPOXY GROUP OPENS UPON REACTION WITH THE ENZYME. THE INHIBITOR HAS TWO C-TERMINI AND NO N TERMINUS.

Cathepsin B (E.C.3.4.22.1), NS-134 inhibitor
Crystal structure of NS-134 in complex with bovine cathepsin B: a two headed epoxysuccinyl inhibitor extends along the whole active site cleft

The largest off-origin peak in the Patterson function is 4.57% of the height of the origin peak. No significant pseudotranslation is detected.

20191225.v01 (using entries in the PDB archive December 25th 2019)

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures