wwPDB validation report of PDB entry 8SMY

Human nucleosome core particle in complex with RNF168 and UbcH5c~Ub

14179 images were recorded in movie-mode of which 14113 were retained for particle picking.

10 mM HEPES, 100 mM NaCl, 1 mM DTT, pH 7.5

Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l

Histone H2A.2,Histone H2A/a,Histone H2A/m

hRNF168,RING finger protein 168,RING-type E3 ubiquitin transferase RNF168

(E3-independent) E2 ubiquitin-conjugating enzyme D3,E2 ubiquitin-conjugating enzyme D3,Ubiquitin carrier protein D3,Ubiquitin-conjugating enzyme E2(17)KB 3,Ubiquitin-conjugating enzyme E2-17 kDa 3,Ubiquitin-protein ligase D3

Cryo-EM structure of the human nucleosome core particle in complex with RNF168 and UbcH5c~Ub (UbcH5c chemically conjugated to histone H2A. No density for Ub.) (class 3)

Calculated fsc threesigma indicator curve
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)