wwPDB validation report of PDB entry 7SBA

Model was built de novo using coot then flexibly refined using ISOLDE.

Type I-D Cascade bound to a dsDNA target

Images were preprocessed and particles were picked via WARP, processing done using 2D classification in cryosparc v2, focused 3D classification in RELION, then Non-Uniform refinement in cryosparc v2.

Monodisperse sample of elongated particles.

Blot force 0, blot time 4.5s, no wait time between sample application and blotting.

CRISPR-Cas type I-D complex bound to a dsDNA target.

Structure of type I-D Cascade bound to a dsDNA target
Author provided fsc threesigma indicator curve
Author provided fsc halfbit indicator curve
Author provided fsc onebit indicator curve
Author provided fsc 0.333 indicator curve
Author provided fsc 0.143 indicator curve
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)