wwPDB validation report of PDB entry 3RWA

CIRCULAR PERMUTATION OF N AND C TERMINAL REGIONS
THE AUTHORS HAVE NOT INTRODUCED ANY MUTATIONS TO THE PROTEIN. THE REPORTED DISCREPANCY IS DUE TO THE FACT THAT THIS PROTEIN HAS BEEN RE-ENGINEERED MANY TIMES BY OTHER LABS FOR ITS APPLICATION IN THE PAST FEW YEARS.

Crystal structure of circular-permutated mKate

The largest off-origin peak in the Patterson function is 5.45% of the height of the origin peak. No significant pseudotranslation is detected.

20191225.v01 (using entries in the PDB archive December 25th 2019)
structure factor analysis and validation
geometric validation package for ligand chemistry in protein x-ray structures