wwPDB validation report of PDB entry 6RR7

Sample was purified in 20 mM HEPES, pH 7.5, 150 mM NaCl with Tween 20 added to a final concentration 0f 0.005% prior to plunging grids.

Sample was treated with 0.001% glutaraldehyde for 20 min on ice prior quenching with 100 mM Tris-HCl pH 7.5 and gel filtration.
Influenza A virus (A/NT/60/1968) polymerase Heterotrimer bound to 3'5' vRNA promoter and Capped RNA

Influenza A 5' and 3' viral RNA promoter

RNA-directed RNA polymerase catalytic subunit

RNA (5'-R(P*GP*CP*CP*UP*GP*CP*UP*UP*UP*UP*G)-3')

RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*C)-3')

Polymerase basic protein 1,PB1,RNA-directed RNA polymerase subunit P1

Influenza A virus (A/NT/60/1968) polymerase Heterotrimer bound to 3'5' vRNA promoter and capped RNA primer

Polymerase acidic protein (E.C.3.1.-.-), RNA-directed RNA polymerase catalytic subunit (E.C.2.7.7.48), Polymerase basic protein 2

Author provided fsc threesigma indicator curve
Author provided fsc halfbit indicator curve
Author provided fsc onebit indicator curve
Author provided fsc 0.333 indicator curve
Author provided fsc 0.143 indicator curve
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)