wwPDB validation report of PDB entry 2REN

THE HUMAN RENIN GENE HAS BEEN SEQUENCED BY TWO GROUPS:
1. HOBART ET AL. (1984) PNAS, V. 81, P. 5026
2. HARDMAN ET AL. (1984) DNA, V. 3, P. 457
THE EXON5-EXON6 JUNCTION IN 2. HAS A 9 BASE EXON CODING FOR
AN ASP-SER-GLU TRIPEPTIDE THAT IS NOT PRESENT IN 1. IN
ADDITION THE C-DNA SEQUENCE OF IMAI ET AL. (1983) PNAS,
V. 80, P. 7405 IS ALSO AVAILABLE AND AGREES WITH THE GENE
SEQUENCE OF HARDMAN ET AL.. THE ELECTRON DENSITY MAP OF
RECOMBINANT HUMAN RENIN IS EXTREMELY POOR IN THIS REGION
AND THE DEPOSITORS COULD NOT RESOLVE THIS DISCREPANCY.
THE COMPLETE LOOP CONTAINING THIS TRIPEPTIDE IS DISORDERED.
RESIDUE NUMBERING IN THIS ENTRY AND IN THE 1989 SCIENCE
PAPER FOLLOWS THE SEQUENTIAL NUMBERING DERIVED FROM THE
SEQUENCE OF HARDMAN ET AL. USED IN THIS ENTRY.

ATOMS IN THE FOLLOWING RESIDUES HAVE BEEN ASSIGNED A TEMPERATURE FACTOR OF 99.99 INDICATING THAT THE ASSOCIATED ELECTRON DENSITY IS VERY POOR: ARG 82 - GLY 86 SER 213 - THR 214 ALA 248 - ASP 254

STRUCTURE OF RECOMBINANT HUMAN RENIN, A TARGET FOR CARDIOVASCULAR-ACTIVE DRUGS, AT 2.5 ANGSTROMS RESOLUTION

20191225.v01 (using entries in the PDB archive December 25th 2019)

geometric validation package for ligand chemistry in protein x-ray structures