wwPDB validation report of PDB entry 1QOL

FMDV LEADER PROTEASE (LBSHORT-C51A-C133S)

FOOT-AND-MOUTH DISEASE VIRUS/ OLIGOSACCHARIDE RECEPTOR COMPLEX.

THE SWISSPROT ENTRY POLG_FMDVO REFERS TO A GENOME POLYPROTEIN, AND THE PROTEASE (EC 3.4.22.-) LISTED IS FOR THE RESIDUES 1650 TO 1862. THE PROTEASE STUDIED HERE WAS FOUND IN THE RESIDUES 29 TO 195 THAT ARE LISTED IN SWISSPROT AS A NONSTRUCTURAL PROTEIN P20A.

NATIVE PATTERSON MAP PRESENT A PSEUDO-ORIGEN PEAK AT POSITION (0.5,0.5,0.225). THIS PEAK CORRESPONDS A PURE TRANSLATION THAT RELATES FOUR MOLECULES OF THE A.S.U. WITH THE OTHER FOUR

BIOCHEMICAL DATA INDICATES THAT LEADER
PROTEASEIS ACTIVE AS A MONOMER. IN THE CRYSTAL
ITASSOCIATES TO FORM A DIMER.

The analyses of the Patterson function reveals a significant off-origin peak that is 43.45 % of the origin peak, indicating pseudo-translational symmetry. The chance of finding a peak of this or larger height by random in a structure without pseudo-translational symmetry is equal to 1.7599e-04. The detected translational NCS is most likely also responsible for the elevated intensity ratio. See the relevant section of the logfile for more details.

20191225.v01 (using entries in the PDB archive December 25th 2019)
structure factor analysis and validation
geometric validation package for ligand chemistry in protein x-ray structures