wwPDB validation report of PDB entry 6QD0

The consensus model was flexibily fit in to the EM map of this class using Normal Mode Analysis with iMODFIT, and then real-space refined using PHENIX.

20 mM KCl, 20 mM Tris-HCl pH 7.6, 1 mM BaCl2, 1 mM EDTA, 0.2 mM cAMP

The defocus at the center of each particle box was estimated based on the tilt geometry.

MloK1 potassium channel in lipidic bilayer of 2D crystals

The dataset of 346 movies recorded and processed for Kowal et al. (2018) 2 was employed. As reported, the data were collected on an FEI Titan Krios TEM equipped with a Gatan K2 DED. Total dose: 40 e-/A^2 distributed over 40 movie frames. Pixel size: 1.3 A on the sample level (counting mode). Nominal tilt range: -55 to +55 degrees.

The particle positions were given by the cross-correlation profile generated by the classical unbending algorithm.

Cyclic nucleotide-gated potassium channel mll3241

MloK1 model from single particle analysis of 2D crystals, class 4 (compact/open conformation)

Calculated fsc threesigma indicator curve
Author provided fsc threesigma indicator curve
Author provided fsc halfbit indicator curve
Author provided fsc onebit indicator curve
Author provided fsc 0.333 indicator curve
Author provided fsc 0.143 indicator curve
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)