wwPDB validation report of PDB entry 1P58

THE RECONSTRUCTION WAS COMPUTED FROM 1691 DENGUE VIRUS IMAGES THAT WERE SELECTED FROM 78 MICROGRAPHS. ORIENTATIONS WERE DETERMINED BY THE MODEL-BASED POLAR-FOURIER TRANSFORM METHOD (BAKER AND CHENG, 1996, J.STRUC.BIOL. 116,120-130) AND REFINED BY THE MODEL-BASED FOURIER TRANSFORM REFINEMENT PROCEDURE(http://bond.cs.ucf.edu/ComputationalBiology/Projects/POR/Home.html).

each viral image was CTF corrected before reconstruction, based on the following equation: F(corr)=F(obs)/[|CTF|+wiener*(1-|CTF|)]

SAMPLES WERE PREPARED AS THIN LAYERS OF VITREOUS ICE AND MAINTAINED AT LIQUID NITROGEN TEMPERATURE IN THE ELECTRON MICROSCOPE

Our previous deposited fitting model of the major membrane protein E based on the 24 Angstrom cryo-EM reconstruction of Dengue virus

Envelope Glycoprotein E From Tick-Borne Encephalitis Virus. The structure of this protein was used to build the homology model of the dengue virus E glycoprotein ecto domain.

Colicin E3. An Antiparallel Coiled-coil segment of this protein was used to fit the transmembrane region of E Glycoprotein.

icosahedral asymmetric unit, std point frame

Major envelope protein E/Envelope glycoprotein M
Complex Organization of Dengue Virus Membrane Proteins as Revealed by 9.5 Angstrom Cryo-EM reconstruction

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)