wwPDB validation report of PDB entry 7NST

Bacteriocin colicinE9 bound to outer membrane protein receptor BtuB and translocator ompF. Disulphide linked to tolB.

data recorded as gain corrected mrc files

3 uL of diluted translocon preparation were applied on freshly glow discharged grids coated with graphene oxide (as described in https://doi.org/10.1038/s41594-019-0355-2); after 30sec waiting grids were blotted for 8-10 using -10 force and plunge frozen in liquid ethane

Outer membrane protein 1A,Outer membrane protein B,Outer membrane protein IA,Porin OmpF

Calculated fsc threesigma indicator curve
Author provided fsc threesigma indicator curve
Author provided fsc halfbit indicator curve
Author provided fsc onebit indicator curve
Author provided fsc 0.333 indicator curve
Author provided fsc 0.143 indicator curve
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)