wwPDB validation report of PDB entry 4ND4

Lactate dehydrogenase, adjacent gene encodes predicted malate dehydrogenase

PEPTIDE SEQUENCE FOR THIS ENTRY WAS TRANSLATED FROM DNA SEQUENCING OF ACTUAL CLONE USED FOR PROTEIN EXPRESSION. AUTHORS DO NOT KNOW IF THE SEQUENCE DIFFERENCES ARE PCR ERRORS OR MUTATIONS CORRESPONDING DNA POLYMORPHISM OR THERE WERE ERRORS IN PROTEIN DATABASE.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Lactate dehydrogenase, adjacent gene encodes predicted malate dehydrogenase (E.C.1.1.1.27)
Crystal structure of the lactate dehydrogenase from cryptosporidium parvum complexed with substrate (pyruvic acid) and cofactor (b-nicotinamide adenine dinucleotide)

The largest off-origin peak in the Patterson function is 3.59% of the height of the origin peak. No significant pseudotranslation is detected.

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)