wwPDB validation report of PDB entry 6N07

The selected particle subset was refined without masking and subsequently masked to reveal only the subregion of the BMC shell to which the focused classification had been applied.

Initial CTF fitting using CTFFIND4, CTF correction applied within RELION

Bacterial microcompartment shell from Haliangium ochraceum

Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2)

928 images were retained after inspection for image quality.

GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER

1000 particles were picked manually to generate reference templates for subsequent auto-picking in RELION 1.4.

5-7 second incubation of the sample on the grid before blotting and plunging

Structure of the HO BMC shell: BMC-TD focused map, open inner pore, compacted shell
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)