wwPDB validation report of PDB entry 6MW3

Glycerol in original storage buffer was diluted to < 0.25% w/v.

Beta subunit core density only visible at low threshold. Beta subunit tail is bound with strong density to alpha subunit and modeled as a poly-A peptide in the model.
Inhibited filament of ribonucleoside-diphosphate reductase composed of NrdE alpha subunits and NrdF beta subunit tails

Cryo-EM samples of the NrdEF filament were prepared by mixing 20 uM C382S holo-NrdE with 20 or 40 uM Mn-reconstituted NrdF in assay buffer with 100 uM dATP and 1 mM CDP, prior to dilution with nucleotide-containing buffer to a concentration of 5 uM protein. A subset of the grids were pre-coated with a support film of continuous, amorphous carbon by flotation of cleaved mica. For these grids, the sample was diluted to a final protein concentration of 2 uM.

C-terminus modeled as a polyalanine chain
Ribonucleoside-diphosphate reductase NrdF beta subunit

6MT9 is the protein in a dimer with an oxidized C-terminus

6MVE is the protein in a dimer with a reduced C-terminus

6MV9 is the same protein in a dimer crystalized with CDP rather than GDP present.

EM structure of Bacillus subtilis ribonucleotide reductase inhibited filament composed of NrdE alpha subunit and NrdF beta subunit with dATP

B. subtilis Ribonucleoside-diphosphate reductase beta subunit (NrdF)
is modeled as an 8-residue polyA chain. The complete sequence is
provided below:
MGSSHHHHHHSSGLVPRGSHMVTKIYDAANWSKHEDDFTQMFYNQNVKQFWLPEEIALNGDLLTWKYL
GKNEQDTYMKVLAGLTLLDTEQGNTGMPIVAEHVDGHQRKAVLNFMAMMENAVHAKSYSNIFMTLAPT
ETINEVFEWVKQNKYLQKKAQMIVGLYKAIQKDDEISLFKAMVASVYLESFLFYSGFYYPLYFYGQGK
LMQSGEIINLILRDEAIHGVYVGLLAQEIYNKQTEEKKAELREFAIDLLNQLYENELEYTEDLYDQVG
LSHDVKKFIRYNANKALMNLGFDPYFEEEDINPIVLNGLNTKTKSHDFFSMKGNGYKKATVEPLKDDD
FYFEDEKEQI

Ribonucleoside-diphosphate reductase (E.C.1.17.4.1), Ribonucleoside-diphosphate reductase beta subunit C-terminal tail

Calculated fsc threesigma indicator curve
Author provided fsc threesigma indicator curve
Author provided fsc halfbit indicator curve
Author provided fsc onebit indicator curve
Author provided fsc 0.333 indicator curve
Author provided fsc 0.143 indicator curve
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)