wwPDB validation report of PDB entry 3LWU

Flat collimating mirror, toroid focusing mirror

Succinylglutamate desuccinylase/aspartoacylase

THIS CONSTRUCT WAS EXPRESSED WITH A PURIFICATION TAG MGSDKIHHHHHHENLYFQG.

1. HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. 2. ATOM RECORDS CONTAIN RESIDUAL B FACTORS ONLY. 3. A MET-INHIBITION PROTOCOL WAS USED FOR SELENOMETHIONINE INCORPORATION DURING PROTEIN EXPRESSION. THE OCCUPANCY OF THE SE ATOMS IN THE MSE RESIDUES WAS REDUCED TO 0.75 FOR THE REDUCED SCATTERING POWER DUE TO PARTIAL S-MET INCORPORATION. 4. ZN HAS BEEN MODELED ON THE BASIS OF ANOMALOUS DIFFERENCE FOURIERS AND ITS USE AS AN ADDITIVE IN THE CRYSTALLIZATION SOLUTION. 5. A SULFATE MOLECULE (SO4) FROM THE CRYSTALLIZATION SOLUTION IS MODELED. 6. AN UNKNOW LIGAND (UNL) HAS BEEN MODELED IN THE ACTIVE SITE NEAR ZN. THE UNL RESEMEBLES A DERIVATIVE OF EITHER SUCCINYL-GLUTAMATE OR GLUTAMATE. 7. RAMACHANDRAN OUTLIER RESIDUE 176 IS WELL SUPPORTED BY THE DENSITY.

Crystal structure of Putative succinylglutamate desuccinylase/aspartoacylase (YP_749235.1) from Shewanella frigidimarinA NCIMB 400 at 2.10 A resolution

CRYSTAL PACKING ANALYSIS SUGGESTS THE ASSIGNMENT OF A TETRAMER AS THE SIGNIFICANT OLIGOMERIZATION STATE.

The largest off-origin peak in the Patterson function is 5.39% of the height of the origin peak. No significant pseudotranslation is detected.

20191225.v01 (using entries in the PDB archive December 25th 2019)

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures