wwPDB validation report of PDB entry 6LTR

DNA (5'-D(*GP*CP*CP*GP*CP*TP*TP*TP*CP*TP*TP*T)-3')

(1) Chain A contains the expression tag SER (0).

(2) The author does not know the exact sequence of chain E.
This short DNA chain is the substrate of Cas12i2 which is a DNA nuclease and it is bound in the catalytic pocket of the protein. The author built the model manually based on the Fo-Fc map and then refined the model. Due to the limited resolution, the author couldn't distinguish between DT and DC, DA and DG.

Crystal structure of Cas12i2 ternary complex with single Mg2+ bound in catalytic pocket

The largest off-origin peak in the Patterson function is 2.72% of the height of the origin peak. No significant pseudotranslation is detected.

20191225.v01 (using entries in the PDB archive December 25th 2019)
structure factor analysis and validation
geometric validation package for ligand chemistry in protein x-ray structures