wwPDB validation report of PDB entry 5J12

The signal peptide (residues 1 - 28) is removed from the mature protein.
N64Q,K126S + residues 127-131 were deleted

Before crystallisation, the N-terminal His-tag (residues 1 - 17, MGSSHHHHHHSSGLVPR) was removed by thrombin cleavage.

Cytokine receptor-like 2,IL-XR,Thymic stromal lymphopoietin protein receptor,TSLP receptor

- rigid body refinement (single rigid body per domain) in Phenix

- Iterative cycles of refinement in autoBuster, model (re)building and validation:
xyz coordinate refinement
individual B-factors
TLS parameterisation with one TLS-group per domain
target restraints:
chain A,C --> chain A,C in the 2.55 Angstrom structure of the human ternary TSLP complex
chain B --> chain B of pdb 2NN5
automatic X-ray weight optimisation
structure validation in COOT, Phenix (Molprobity) and PDB_REDO

Thymic stromal lymphopoietin, Interleukin-7 receptor subunit alpha, Cytokine receptor-like factor 2
Structure of human TSLP:TSLPR in complex with mouse IL-7Ralpha

The largest off-origin peak in the Patterson function is 2.97% of the height of the origin peak. No significant pseudotranslation is detected.

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)