wwPDB validation report of PDB entry 6GSA

"Fit in map" function used to place 6FE8 and 4ACO with out further refinement or model building

The truncated CBF3 complex, recombinantly expressed in Saccharomyces cerevisiae. It comprises a Cep3 homodimer, in which the binuclear zinc cluster domains are truncated, full length heterodimer of Skp1 and Ctf13, and a monomeric construct Ndc10 comprising domains 1-2.

The selected images were high-pass filtered and normalized.

Number of particles after selection by 2D classification

CTF parameters were estimated using CTFFIND4

The sample is homogeneous and well-dispersed on grids.

N-terminal polyhistidine purification tagTruncation of the binuclear zinc cluster domain
Centromere DNA-binding protein complex CBF3 subunit B
Truncation of the N-terminal domain, UNP residues 1-46

Centromere DNA-binding protein complex CBF3 subunit C

Domains 1-2 of Ndc10 with a non-cleavable C-terminal StrepII tag
Centromere DNA-binding protein complex CBF3 subunit A
Construct comprising residues 1-554 with C-terminal Strep tag

Centromere DNA-binding protein complex CBF3 subunit D,E3 ubiquitin ligase complex SCF subunit SKP1

Chromosome transmission fidelity protein 13,Kinetochore protein CTF13

Centromere-binding factor 2,Chromosome transmission fidelity protein 14,Kinetochore protein CTF14

The structure of the CBF3 core complex within the larger CBF3

Core Centromere Binding Factor 3 (CBF3) with monomeric Ndc10

Centromere DNA-binding protein complex CBF3 subunit B, Suppressor of kinetochore protein 1, Centromere DNA-binding protein complex CBF3 subunit C
Author provided fsc threesigma indicator curve
Author provided fsc halfbit indicator curve
Author provided fsc onebit indicator curve
Author provided fsc 0.333 indicator curve
Author provided fsc 0.143 indicator curve
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)