wwPDB validation report of PDB entry 3G4T

1.9 Angstrom structure of the thermophilic exonuclease III homologue Mth0212

Mth0212 in complex with a 9bp blunt end dsDNA at 1.7 Angstrom

Complex of Mth0212 and an 8bp dsDNA with distorted ends

Mth0212 (WT) crystallized in a monoclinic space group

Mth0212 in complex with a short ssDNA (CGTA)

Complex of Mth0212 and a 4 bp dsDNA with 3'-overhang

The catalytically inactive mutant Mth0212 (D151N) in complex with an 8 bp dsDNA

Mth0212 (WT) in complex with a 6bp dsDNA containing a single one nucleotide long 3'-overhang

Mth0212 in complex with ssDNA in space group P32

The rationally designed catalytically inactive mutant Mth0212(D151N)

1.2 Angstrom structure of the exonuclease III homologue Mth0212

THE 3'-END OF BOTH CHAIN I IS BOUND TO ROX 5/6 NHS MIXTURE (FLUORESCENCE DYE), WHICH IS NOT SHOWN IN THIS ENTRY.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS; MOST OF THE TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS, FALL INTO GENERALLY ALLOWED REGIONS, ONLY A MINORITY BELONGS TO THE DISALLOWED REGIONS.

Exodeoxyribonuclease (E.C.3.1.11.2)/DNA complex
Mth0212 (WT) in complex with a 7bp dsDNA

The largest off-origin peak in the Patterson function is 5.21% of the height of the origin peak. No significant pseudotranslation is detected.

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)