wwPDB validation report of PDB entry 6FQQ

N-terminal 'GP' sequence comes from the purification tag the protein construct corresponds to a double mutant: R167A/R168A

DNA (5'-D(P*AP*TP*TP*GP*AP*CP*AP*GP*CP*TP*GP*TP*CP*AP*AP*T)-3')

6FQP contains the WT form of the protein

Crystal structure of TALE homeobox domain transcription factor TGIF1 double alanine mutant bound to its consensus DNA

(chain 'A' and (resid 170 through 176 or (resid 177 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 178 through 179 or (resid 180 and (name N or name CA or name C or name O or name CB )) or resid 181 through 184 or (resid 185 and (name N or name CA or name C or name O or name CB )) or resid 186 through 193 or (resid 194 through 195 and (name N or name CA or name C or name O or name CB )) or resid 196 through 204 or (resid 205 and (name N or name CA or name C or name O or name CB )) or resid 206 through 224 or (resid 225 and (name N or name CA or name C or name O or name CB )) or resid 226 through 228 or (resid 229 and (name N or name CA or name C or name O or name CB or name CG ))))

(chain 'B' and (resid 170 through 172 or (resid 173 through 174 and (name N or name CA or name C or name O or name CB )) or resid 175 through 179 or (resid 180 and (name N or name CA or name C or name O or name CB )) or resid 181 through 193 or (resid 194 through 195 and (name N or name CA or name C or name O or name CB )) or resid 196 through 201 or (resid 202 and (name N or name CA or name C or name O or name CB )) or resid 203 through 229))

(chain 'D' and (resid 170 through 172 or (resid 173 through 174 and (name N or name CA or name C or name O or name CB )) or resid 175 through 176 or (resid 177 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 178 through 179 or (resid 180 and (name N or name CA or name C or name O or name CB )) or resid 181 through 184 or (resid 185 and (name N or name CA or name C or name O or name CB )) or resid 186 through 201 or (resid 202 and (name N or name CA or name C or name O or name CB )) or resid 203 through 204 or (resid 205 and (name N or name CA or name C or name O or name CB )) or resid 206 through 224 or (resid 225 and (name N or name CA or name C or name O or name CB )) or resid 226 through 227 or (resid 228 and (name N or name CA or name C or name O or name CB )) or (resid 229 and (name N or name CA or name C or name O or name CB or name CG ))))

(chain 'E' and (resid 170 through 172 or (resid 173 through 174 and (name N or name CA or name C or name O or name CB )) or resid 175 through 176 or (resid 177 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 178 through 184 or (resid 185 and (name N or name CA or name C or name O or name CB )) or resid 186 through 193 or (resid 194 through 195 and (name N or name CA or name C or name O or name CB )) or resid 196 through 201 or (resid 202 and (name N or name CA or name C or name O or name CB )) or resid 203 through 204 or (resid 205 and (name N or name CA or name C or name O or name CB )) or resid 206 through 224 or (resid 225 and (name N or name CA or name C or name O or name CB )) or resid 226 through 228 or (resid 229 and (name N or name CA or name C or name O or name CB or name CG ))))

The analyses of the Patterson function reveals a significant off-origin peak that is 33.57 % of the origin peak, indicating pseudo-translational symmetry. The chance of finding a peak of this or larger height by random in a structure without pseudo-translational symmetry is equal to 7.8349e-04. The detected translational NCS is most likely also responsible for the elevated intensity ratio. See the relevant section of the logfile for more details.

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)