wwPDB validation report of PDB entry 1FLO

CMPOUND
This entry contains the crystallographic
asymmetric unit which contains a tetramer of Flp protein
bound to a DNA Holliday junction that mimics the reaction
intermediate. This is the biologically relevant
multimer.

FLP RECOMBINASE/SYMMETRIZED FRT DNA COMPLEX
FLP Recombinase-Holliday Junction Complex I

The biological assembly is a Flp tetramer bound to a DNA Holliday junction, as constructed by all the chains in one asymmetric unit

ACTIVE CATALYTIC SITE OF MONOMER A. Y343 IS DONATED BY MONOMER D.

ACTIVE CATALYTIC SITE OF MONOMER B. Y343 IS DONATED BY MONOMER C.

INACTIVE CATALYTIC SITE OF MONOMER C. Y343 IS DONATED BY MONOMER A.

INACTIVE CATALYTIC SITE OF MONOMER D. Y343 IS DONATED BY MONOMER B, IS DISORDERED.

The largest off-origin peak in the Patterson function is 3.31% of the height of the origin peak. No significant pseudotranslation is detected.

20191225.v01 (using entries in the PDB archive December 25th 2019)
structure factor analysis and validation
geometric validation package for ligand chemistry in protein x-ray structures