wwPDB validation report of PDB entry 3FDB

Flat collimating mirror, toroid focusing mirror

putative PLP-dependent beta-cystathionase

THE CONSTRUCT WAS EXPRESSED WITH A PURIFICATION TAG MGSDKIHHHHHHENLYFQG. THE TAG WAS REMOVED WITH TEV PROTEASE LEAVING ONLY A GLYCINE (0) FOLLOWED BY THE TARGET SEQUENCE.

1.HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. 2.ATOM RECORD CONTAINS RESIDUAL B FACTORS ONLY. 3.A MET-INHIBITION PROTOCOL WAS USED FOR SELENOMETHIONINE INCORPORATION DURING PROTEIN EXPRESSION. THE OCCUPANCY OF THE SE ATOMS IN THE MSE RESIDUES WAS REDUCED TO 0.75 TO ACCOUNT FOR THE REDUCED SCATTERING POWER DUE TO PARTIAL S-MET INCORPORATION. 4.SODIUM ION, ETHYLENE GLYCOL MOLECULES AND CHLORIDE IONS FROM CRYSTALLIZATION AND CRYOPROTECTANT ARE MODELED INTO THISSTRUCTURE, RESPECTIVELY. 5.ONE PLP MOLECULE FROM PROTEIN EXPRESSION ARE MODELED IN THIS STRUCTURE WITH CLEAR ELECTRON DENSITY SUPPORT.

Crystal structure of a putative plp-dependent beta-cystathionase (aecd, dip1736) from corynebacterium diphtheriae at 1.99 A resolution

The largest off-origin peak in the Patterson function is 4.42% of the height of the origin peak. No significant pseudotranslation is detected.

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)