wwPDB validation report of PDB entry 6EZN

yeast oligosaccharyltransferase (OST) complex

Quantifoil carbon grids (300 mesh, copper) were glow discharged at 25 mA for 40 s. 3.5 ul of nanodisc-reconstituted OST sample at concentration of 0.5 mg/ml was applied onto the grid and grids were blotted for 3 s and flash-frozen in a mixture of liquid ethane and propane cooled by liquid nitrogen.

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1

Oligosaccharyl transferase 64 kDa subunit,Oligosaccharyl transferase subunit OST1,Oligosaccharyl transferase subunit alpha

Oligosaccharyl transferase subunit OST2,Oligosaccharyl transferase 16 kDa subunit,Oligosaccharyl transferase subunit epsilon

Oligosaccharyl transferase 34 kDa subunit,Oligosaccharyl transferase subunit OST3,Oligosaccharyl transferase subunit gamma

Oligosaccharyl transferase subunit OST4,Oligosaccharyl transferase 4 kDa subunit,OTase 4 kDa subunit

Oligosaccharyl transferase subunit OST5,Oligosaccharyl transferase 9.5 kDa subunit,Oligosaccharyl transferase subunit zeta

Oligosaccharyl transferase subunit WBP1,Oligosaccharyl transferase subunit beta

Oligosaccharyl transferase subunit SWP1,Oligosaccharyl transferase subunit delta

Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex

Amino acid residues H22 to H65 in Swp1 were built as PolyA chain and renamed to specific amino acids according to residue numbers on request by wwPDB

Author provided fsc threesigma indicator curve
Author provided fsc halfbit indicator curve
Author provided fsc onebit indicator curve
Author provided fsc 0.333 indicator curve
Author provided fsc 0.143 indicator curve
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)