wwPDB validation report of PDB entry 8EH0

engineered from tryptophan synthase beta chain 1

2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid

The starting construct was tryptophan synthase beta chain 1, which was engineered to produce the construct used for expression and crystallization.

Engineered tyrosine synthase (TmTyrS1) derived from T. maritima TrpB with Ser bound as the amino-acrylate intermediate and complexed with quinoline N-oxide

The largest off-origin peak in the Patterson function is 4.02% of the height of the origin peak. No significant pseudotranslation is detected.

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)