wwPDB validation report of PDB entry 1EAQ

ENGINEERED MUTATION CYS 72 SER, CYS 81 SER

ACUTE MYELOID LEUKEMIA 1 PROTEIN, ONCOGENE AML-1, CORE-BINDING FACTOR, ALPHA B SUBUNIT, CBF-ALPHA B, POLYOMAVIRUS ENHANCER BINDING PROTEIN 2 ALPHA B SUBUNIT, PEBP2-ALPHA B, PEA2-ALPHA B, SL3-3 ENHANCER FACTOR 1 ALPHA B SUBUNIT, SL3/AKV CORE-BINDING FACTOR ALPHA B SUBUNIT

IMMUNOGLOBULIN MOTIF DNA-RECOGNITION AND HETERODIMERIZATION FOR THE PEBP2/CBF RUNT-DOMAIN

AN "S-SWITCH" AND CHLORIDE IONS MODULATE THE DNA-BINDING ABILITY OF RUNX/AML1

CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN AND C/EBPBETA BZIP DIMERIC BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER

CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER

CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN-CBFBETA CORE DOMAIN HETERODIMER AND C/EBPBETA BZIP DIMERIC BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER

CHAIN A ENGINEERED MUTATION CYS 72 SER, CYS 81 SER
CHAIN B ENGINEERED MUTATION CYS 72 SER, CYS 81 SER

CBF BINDS TO THE CORE SITE, OF A NUMBER OF ENHANCERS AND PROMOTERS,
INCLUDING MURINE LEUKEMIA VIRUS, POLYOMAVIRUS ENHANCER,
T-CELL RECEPTOR ENHANCERS, LCK, IL-3 AND GM-CSF PROMOTERS.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

The RUNX1 Runt domain at 1.25A resolution: A structural switch and specifically bound chloride ions modulate DNA binding

The largest off-origin peak in the Patterson function is 7.57% of the height of the origin peak. No significant pseudotranslation is detected.

20191225.v01 (using entries in the PDB archive December 25th 2019)

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures