wwPDB validation report of PDB entry 1E5G

SP35,28 KDA PROTEIN, SECRETORY PROTEIN 35, PROTEIN C3, VCP

C-TERMINAL HALF OF VACCINIA VIRUS COMPLEMENT CONTROL PROTEIN, NMR, MINIMIZED AVERAGE STRUCTURE

C-TERMINAL HALF OF VACCINIA VIRUS COMPLEMENT CONTROL PROTEIN, NMR, 21 STRUCTURES

FUNCTION: SERVES TO PROTECT THE VIRUS AGAINST COMPLEMENT ATTACK BY
INHIBITING BOTH CLASSICAL AND ALTERNATIVE PATHWAYS OF COMPLEMENT
ACTIVATION. BINDS C3B AND C4B.
SIMILARITY: BELONGS TO THE SUPERFAMILY OF THE REGULATORS OF
COMPLEMENT ACTIVATION (RCA).
SIMILARITY: CONTAINS 4 SUSHI (SCR) REPEATS.

15N-LABELLED VCP MODULES 2 AND 3 WAS USED TO OBTAIN HSQC NOESY AND TOCSY EXPERIMENTS TO ASSIGN THE BACKBONE ATOMS. 2D EXPERIMENTS WERE THEN USED IN CONJUNCTION WITH THE 3D TECHNIQUES TO ASSIGN THE SIDE CHAIN ATOMS AND THE STRUCTURE WAS CALCULATED USING UNAMBIGUOUS AND AMBIGUOUS RESTRAINTS

REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION

Solution structure of central CP module pair of a pox virus complement inhibitor

20191225.v01 (using entries in the PDB archive December 25th 2019)
geometric validation package for ligand chemistry in protein x-ray structures
assessment of nmr assigned chemical shifts
geometric validation of nmr ensemble structure
domain identification of nmr ensemble structure
classification of nmr ensemble structure
nmr chemical shift validation and reference correction
random coil index prediction from nmr chemical shifts