wwPDB validation report of PDB entry 1E2W

N153Q MUTANT OF CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII

Q158L MUTANT OF CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII

WILD TYPE CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII

LUMEN-SIDE DOMAIN OF REDUCED CYTOCHROME F AT -35 DEGREES CELSIUS (BRASSICA RAPA)

CYTOCHROME F FROM THE B6F COMPLEX OF PHORMIDIUM LAMINOSUM

STRUCTURE OF CYTOCHROME F FROM CHLAMYDOMONAS REINHARDTII

CHAIN A, B, C ENGINEERED MUTATION ASN168PHE. ONLY THE SOLUBLE DOMAIN OF THE MATURE MEMBRANE PROTEIN WAS EXPRESSED AND CRYSTALLIZED (251 A.A. OF 317 A.A.). 31-RESIDUE PRE-SEQUENCE IS A THYLAKOID IMPORT SIGNAL C-TERMINAL 35-RESIDUES ARE THE MEMBRANE ANCHOR. BOTH WERE EXCLUDED FROM THE EXPRESSED CONSTRUCT.

THE LOOP 186-190 IS BADLY DEFINED IN THE ELECTRON DENSITY. ONLY THE MAIN CHAIN OF THE ARG 251 WAS SEEN IN THE ELCTRON DENSITY MAP FOR THE B CHAIN , SO AN ALA 251 WAS USED

N168F mutant of cytochrome f from Chlamydomonas reinhardtii

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)