wwPDB validation report of PDB entry 5DUW

N-terminal carbohydrate recognition domain (UNP residues 1-155)

Gal-4,Antigen NY-CO-27,L-36 lactose-binding protein,L36LBP,Lactose-binding lectin 4

The authors state that the electron density currently modelled as water molecules A304, B302, C301 and D301, which are coordinated by residues ASP72, GLY70 and PHE68 shows potential for occupation by NA+ ions, which is present in the crystallisation conditions.

Crystal structure of the human galectin-4 N-terminal carbohydrate recognition domain in complex with lactose-3'-sulfate

The analyses of the Patterson function reveals a significant off-origin peak that is 30.89 % of the origin peak, indicating pseudo-translational symmetry. The chance of finding a peak of this or larger height by random in a structure without pseudo-translational symmetry is equal to 1.2124e-03. The detected translational NCS is most likely also responsible for the elevated intensity ratio. See the relevant section of the logfile for more details.

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)