wwPDB validation report of PDB entry 2DRC

RESIDUE 154 IS GLU IN THE RT500 STRAIN. IN THE MB1428 STRAIN (PROTEIN DATA BANK ENTRY 4DFR), THIS RESIDUE IS A LYS.

THE MOLECULE DESIGNATED AS CHAIN B BELOW IS PREFERRED FOR STRUCTURAL COMPARISONS BECAUSE IT IS MORE COMPLETE AND LESS PERTURBED BY INTERMOLECULAR CONTACTS.

DIHYDROFOLATE REDUCTASE (DHFR) (E.C.1.5.1.3) MUTANT WITH TRP 22 REPLACED BY PHE (W22F)
INVESTIGATION OF THE FUNCTIONAL ROLE OF TRYPTOPHAN-22 IN ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE BY SITE-DIRECTED MUTAGENESIS

THE TRANSFORMATION PRESENTED ON *MTRIX* RECORDS BELOW WILL YIELD APPROXIMATE COORDINATES FOR CHAIN *A* WHEN APPLIED TO CHAIN *B*.

RESIDUES INTERACTING WITH THE PTERIDINE OF THE METHOTREXATE INHIBITOR. INCLUDE WATER MOLECULES WHICH ARE BOUND EITHER TO INVARIANT SIDE CHAINS OR TO STRUCTURALLY INVARIANT MAIN CHAIN SEGMENTS.

RESIDUES INTERACTING WITH THE N(10) METHYL OF THE METHOTREXATE INHIBITOR

RESIDUES INTERACTING WITH THE P-AMINO BENZOYL OF THE METHOTREXATE INHIBITOR

RESIDUES INTERACTING WITH THE GLUTAMATE OF THE METHOTREXATE INHIBITOR

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)