wwPDB validation report of PDB entry 6DDE

Solutions were made fresh. After buffer reconstitution, all buffers were filtered with 0.22um filter.

Signaling complex formed by incubation of DAMGO-bound Mu-type opioid receptor and heterotrimeric Gi. Excess GDP removed by addition of apyrase. ScFv added to stabilize complex.
Ternary complex of DAMGO-activated Mu-type opioid receptor with heterotrimeric Gi, further stabilized by addition of Scfv-16

Images were collected in movie-mode at 10 frames per second.

This sample was monodisperse by negative stain and Cryo-EM analysis.

Guanine nucleotide-binding protein G(i) subunit alpha-1

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Adenylate cyclase-inhibiting G alpha protein

Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, scFv16, Mu-type opioid receptor, DAMGO

geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)