wwPDB validation report of PDB entry 8AYZ

Initial model was rigid body fitted using UCSF chimera and Coot. Global minimization and B-factor refinement was performed in real space using phenix_real.space.refine.

Final reconstruction was Ewald sphere corrected and sharpened with Post-processing in RELION using an inverse B-factor of -15.0 Angstroms. B-factor was selected ad-hoc while testing a range of values for map interpretability.

1 x DPBS was made from tissue culture grade Dulbecco's Phosphate Buffered Saline (Sigma-Aldrich). 20 mM EDTA was prepared from fresh stocks of buffered EDTA.

Recombinantly expressed virus-like particle of PV2 (strain MEF-1)

All frames were used for motion correction.

Images were collected in electron counting mode with a calibrated pixel size of 0.829 A/pixel. Super-resolution pixel size 0.4145 A/pixel. Movies were collected in 50 frames.

Particle selection was automated with ETHAN particle picking software.

Thermo Fisher Scientific EPU software was used for data collection

CTFFIND4 in RELION was used for CTF correction

Recombinant VLP of PV2 mixed with capsid inhibitor compound 17 at a molar ratio of 1:2500.

Single blotting with 3.5ul of sample. Four second blot time and -17 blot force on the vitrobot.

Sequence is given for the VP0 polypeptide.

4-[[4-[1,3-bis(oxidanylidene)isoindol-2-yl]phenyl]sulfonylamino]benzoic acid

Poliovirus type 2 (strain MEF-1) virus-like particle in complex with capsid binder compound 17

Calculated fsc threesigma indicator curve
Author provided fsc threesigma indicator curve
Author provided fsc halfbit indicator curve
Author provided fsc onebit indicator curve
Author provided fsc 0.333 indicator curve
Author provided fsc 0.143 indicator curve
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)