wwPDB validation report of PDB entry 3AYX

Membrane-bound hydrogenase large subunit

Membrane-bound hydrogenase small subunit

MEMBRANE-BOUND RESPIRATORY NI-FE HYDROGENASE LARGE SUBUNIT

MEMBRANE-BOUND RESPIRATORY NI-FE HYDROGENASE SMALL SUBUNIT

Membrane-bound respiratory [NiFe] hydrogenase from Hydrogenovibrio marinus in a ferricyanide-oxidized condition

Membrane-bound respiratory [NiFe] hydrogenase from Hydrogenovibrio marinus in an air-oxidized condition

THE NI IONS IN THIS STRUCTURE CAN EITHER BE NI(II) OR NI(III) IONS

1. The SF file contains friedel pairs. Friedel pairs were independently treated during refinement.
Statistics for data collection were calculated from data of which the friedel pairs were merged.
2. ANISOTROPIC SCALING APPLIED BY THE METHOD OF PARKIN, MOEZZI & HOPE, J.APPL.CRYST.28(1995)53-56 ANISOTROPIC REFINEMENT REDUCED FREE R (NO CUTOFF) BY ?

Membrane-bound hydrogenase large subunit (E.C.1.12.5.1), Membrane-bound hydrogenase small subunit (E.C.1.12.5.1)
Membrane-bound respiratory [NiFe] hydrogenase from Hydrogenovibrio marinus in an H2-reduced condition

The largest off-origin peak in the Patterson function is 4.19% of the height of the origin peak. No significant pseudotranslation is detected.
structure factor analysis and validation
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)