wwPDB validation report of PDB entry 3AG2

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Cytochrome c oxidase subunit 5A, mitochondrial

Cytochrome c oxidase subunit 5B, mitochondrial

Cytochrome c oxidase subunit 6A2, mitochondrial

Cytochrome c oxidase subunit 7A1, mitochondrial

Cytochrome c oxidase subunit 7B, mitochondrial

Cytochrome c oxidase subunit 7C, mitochondrial

Cytochrome c oxidase subunit 8B, mitochondrial

(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE

(7R,17E,20E)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSA-17,20-DIEN-1-AMINIUM 4-OXIDE

(1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(STEAROYLOXY)METHYL]ETHYL (5E,8E,11E,14E)-ICOSA-5,8,11,14-TETRAENOATE

Cytochrome c oxidase polypeptide IV,Cytochrome c oxidase subunit IV isoform 1,COX IV-1

Cytochrome c oxidase polypeptide VIa,Cytochrome c oxidase polypeptide Vb

Cytochrome c oxidase polypeptide VIa-heart,COXVIAH,Cytochrome c oxidase polypeptide VIb

Cytochrome c oxidase polypeptide VII,Cytochrome c oxidase subunit AED,Cytochrome c oxidase subunit VIb isoform 1,COX VIb-1

Cytochrome c oxidase polypeptide VIc,Cytochrome c oxidase subunit STA

Cytochrome c oxidase subunit VIIIc,VIIIC,Cytochrome c oxidase subunit VIIa-heart,Cytochrome c oxidase subunit VIIa-H,Cytochrome c oxidase subunit VIIa-muscle,Cytochrome c oxidase subunit VIIa-M

Cytochrome c oxidase polypeptide VIIb,IHQ

Cytochrome c oxidase polypeptide VIIIA,Cytochrome c oxidase polypeptide VIIc

Cytochrome c oxidase polypeptide VIII-heart,Cytochrome c oxidase subunit 8-1,Cytochrome c oxidase subunit 8H,IX,VIIIb

Fully reduced CcO crystals were kept in the solution saturated with CO-gass to prepare CO-bound fully reduced crystals. Previous structure analysis had been done under the full occupancy of CO in the Fea3-CuB site. In the present study the CO occupancy was inspected and it was ~60%. Thus the crystal contained two components of the fully reduced CcO and the CO-bound fully reduced CcO. The crystal structure containing multiple structures was determined in the present study.

(chain A and resid 1 through 514) or (chain B
and resid 1 through 227) or (chain C and
resid 3 through 261) or (chain D and resid 4
through 147) or (chain E and resid 5 through
109) or (chain F and resid 1 through 98) or
(chain G and resid 1 through 84) or (chain H
and resid 7 through 85) or (chain I and resid
1 through 73) or (chain J and resid 1 through
58) or (chain K and resid 6 through 54) or
(chain L and resid 2 through 47) or (chain M
and resid 1 through 43)

(chain N and resid 1 through 514) or (chain O
and resid 1 through 227) or (chain P and
resid 3 through 261) or (chain Q and resid 7
through 147) or (chain R and resid 5 through
109) or (chain S and resid 1 through 95) or
(chain T and resid 1 through 84) or (chain U
and resid 7 through 85) or (chain V and resid
1 through 73) or (chain W and resid 1 through
58) or (chain X and resid 6 through 54) or
(chain Y and resid 2 through 47) or (chain Z
and resid 1 through 43)

Bovine Heart Cytochrome c Oxidase in the Carbon Monoxide-bound Fully Reduced State at 100 K
The largest off-origin peak in the Patterson function is 4.39% of the height of the origin peak. No significant pseudotranslation is detected.