wwPDB validation report of PDB entry 6AFP

N136D is isolated from bacterial colonies that resisted to antibiotic, thus the mutation was not actually engineered using artificial method like site-directed mutagenesis.

Crystal structure of class A b-lactamase, PenL, variant Asn136Asp, from Burkholderia thailandensis, in complex with ceftazidime-like boronic acid

The largest off-origin peak in the Patterson function is 6.66% of the height of the origin peak. No significant pseudotranslation is detected.

structure factor analysis and validation

geometric validation package for ligand chemistry in protein x-ray structures

20191225.v01 (using entries in the PDB archive December 25th 2019)