wwPDB validation report of PDB entry 12E8

AMINO ACIDS ARE NUMBERED ACCORDING TO THE KABAT NUMBERING SYSTEM FOR ANTIBODIES.

BULK SOLVENT MODEL USED. THE CONNECTING LOOP BETWEEN BETA STRANDS B AND C IN THE CH1 DOMAIN IS POORLY DEFINED IN THE STRUCTURE. ATTEMPTS TO IMPROVE THE LOOP H123-H133 USING VARIOUS NCS AVERAGING SCHEMES AS WELL AS AUTOMATED REBUILDING AND REFINEMENT WITH ARP DID NOT IMPROVE THIS REGION. HOWEVER, USING ARP THE TRACING OF CH BETA STRANDS B-E WAS CONFIRMED INDEPENDENT OF ANY STARTING MODEL.

THE CONNECTING LOOP BETWEEN BETA STRANDS B AND C IN THE CH1 DOMAIN IS POORLY DEFINED IN THE STRUCTURE. ATTEMPTS TO IMPROVE THE LOOP H123-H133 USING VARIOUS NCS AVERAGING SCHEMES AS WELL AS AUTOMATED REBUILDING AND REFINEMENT WITH ARP DID NOT IMPROVE THIS REGION. HOWEVER, USING ARP THE TRACING OF CH BETA STRANDS B-E WAS CONFIRMED INDEPENDENT OF ANY STARTING MODEL.

The largest off-origin peak in the Patterson function is 11.38% of the height of the origin peak. No significant pseudotranslation is detected.

structure factor analysis and validation
geometric validation package for ligand chemistry in protein x-ray structures
20191225.v01 (using entries in the PDB archive December 25th 2019)