Crystal structure of monkey TLR7 in complex with GGUUGG

PEG 8000, ammonium sulfate, sodium citrate pH 5.0, Tris-HCl pH 7.5 and NaCl

The residues 440-445 (SEVGFC) are replaced by a thrombin-cleavage sequence (LVPRGS) for artificial cleavage of Z-loop. This engineered site in Z-loop was further cleaved during purification and the cleaved protein remained associated via interactions between LRRs and a disulfide bond. Therefore, although cleaved, the cleaved version of protein is considered as one single component (chain).

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Toll-like receptor 7,Toll-like receptor 7 extracellular domain/RNA Complex

Innate immunity Toll-like receptors, IMMUNE SYSTEM