Methanosaeta concilii ATP citrate lyase (D541A mutant) in complex with (3S)-citryl-CoA.

(2~{S})-2-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-2-oxidanylidene-ethyl]-2-oxidanyl-butanedioic acid

Acetyl-CoA is produced by the citrate synthase homology module of ATP-citrate lyase.

Towards automated crystallographic structure refinement with phenix.refine.

PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)

M. concilii ACLY-B fused to a C-terminal hexahistidine tag

0.02 M xylitol
0.02 M myo-inositol
0.02 M D-(-)-fructose
0.02 M L-rhamnose monohydrate
0.02 M D-sorbitol
0.1 M BES/Triethanolamine pH 7.5
12.5 % PEG4000
20 % 1,2,6-hexanetriol

acetyl-CoA production, metabolism, atp citrate lyase, lipogenesis, LYASE