The atomic structure of the HAdVF-41 penton base in solution

The structure of enteric human adenovirus 41-A leading cause of diarrhea in children.

per-micrograph CTF correction as well the initial per-particle CTF correction before 2D classification were performed in GCTF. Later per-particle CTF-refinement were performed in RELION 3.1-beta.

The HAdVF-41 penton base was recombinantely expressed in Sf9 cells.

Data were collected at a 30 degree tilt of the specimen stage due to a preferred orientation of the sample in the vitrified ice.

per-micrograph and initial per-particle CTF correction

The sample was monodisperse with a preferred orientation in the vitrious ice.

HAdVF-41, penton base, pentamer, VIRAL PROTEIN