SpCas9 bound to 18-nucleotide complementary DNA substrate in the checkpoint state

R-loop formation and conformational activation mechanisms of Cas9.

Ternary complex of SpCas9-sgRNA bound to a 18-nucleotide complementary DNA substrate in the inactive state

CRISPR-associated endonuclease Cas9/Csn1

Target strand of 18-nucleotide complementary DNA substrate

Non-target strand of 18-nucleotide complementary DNA substrate

SpCas9 bound to 12-nucleotide complementary DNA substrate

CRISPR, Cas9, R-loop, substrate binding, off-target, HYDROLASE