CryoEM structure of the Chikungunya virus nsP1 complex

Capping pores of alphavirus nsP1 gate membranous viral replication factories.

CTF was estimated from movie frames aligned without dose-weighting. Per particle CTF correction was also performed with polished particles.

Chikungunya virus strain S27-African prototype

Baculovirus expression vector pFastBac1-HM

Autopicking following generation of templates from 2D class averages

3ul of sample was spotted onto the grid and hand blotted prior to plunge freezing.

Capping enzyme, monotopic membrane complex, membrane bending, membrane pore, replication complex, scaffold, Spherule formation, viral factory., VIRAL PROTEIN